香叶木素对人肺腺癌细胞系H1299和A549增殖能力影响的研究

宋长山; 沈柏儒; 朱珊; 苏珊; 陈家亿; 朱尚媚; 余敏

doi:10.13201/j.issn.1009-5918.2017.04.013

香叶木素对人肺腺癌细胞系H1299和A549增殖能力影响的研究

- 1. 佛山市禅城区中心医院(广东医学院附属禅城医院)胸外科(广东佛山, 528000);
- 2. 佛山市禅城区中心医院(广东医学院附属禅城医院)妇产科
基金项目:
2016年佛山市科技局科技攻关项目 (No:2016AB001691)

详细信息

通讯作者: 沈柏儒, E-mail:bairushen@yeah.net

中图分类号: R734.2

收稿日期: 2017-02-06

The effect of the diosmetin on growth and proliferation of human lung adenocarcinoma cell line H1299 and A549 in vitro

- 1 Department of Thoracic Surgery, Chancheng District Central Hospital of Foshan City, Foshan 528000, China;
- 2 Department of Obstetrics and Gynecology, Chancheng District Central Hospital of Foshan City

More Information

Corresponding author: SHEN Bairu, E-mail: bairushen@yeah.net

Received Date: 06 February 2017

摘要

摘要: 目的: 探究香叶木素(Dios)作用前后人肺腺癌细胞系H1299和A549细胞增殖活性的改变。方法: 取RPMI-1640培养基培养的人肺腺癌细系H1299及A549用于实验。采用CCK8法检测Dios作用前后人肺腺癌细胞系H1299和A549细胞增殖活性的改变。结果: 不同浓度(0、5、10、20 μmol/L)Dios作用H1299细胞24 h后,细胞的增殖抑制率分别为(10.9±2.5)%、(32.7±1.8)%、(41.2±2.1)%、(51.7±2.8)%,而A549细胞增殖抑制率为(18.9±2.3)%、(30.8±2.6)%、(42.2±1.2)%、(50.7±2.1)%。结论: Dios对人肺腺癌细胞系H1299和A549的增殖有明显的抑制作用,提示Dios通过抑制肺腺癌细胞的增殖,进而起到良好的抗肿瘤作用。
- 香叶木素 /
- 肺腺癌 /
- 增殖
Abstract: Objective: To investigate the changes of proliferation activity in human lung adenocarcinoma cell lines H1299 and A549 before and after intervention with diosporine (Dios). Method: Human lung adenocarcinoma cell lines H1299 and A549 cultured in PRIM-1640 medium were used in the experiment.We used the CCK8 methods to detect the proliferation activity of human lung adenocarcinoma cell lines H1299 and A549 before and after intervention with Dios. Result: H1299 cells were treated with Dios at differentconcentrations (0,5,10,20 μmol/L) for 24 h,the cell proliferation inhibition rate was (10.9±2.5)%,(32.7±1.8)%,(41.2±2.1)%,(51.7±2.8)%,respectively;while the cell proliferation inhibition rate in A549 cells was (18.9±2.3)%,(30.8±2.6)%,(42.2±1.2)%,(50.7±2.1)%,respectively. Conclusion: Dios has an obvious inhibitory effect on the proliferation in human lung adenocarcinoma cell lines H1299 and A549,suggesting that Dios inhibits the proliferation of lung adenocarcinoma cells and thus plays a good anti-tumor effect.
- diosmetin /
- lung adenocarcinoma /
- proliferation

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