Effect of sodium tanshinone Ⅱ A sulfonate on the inflammatory reaction in cardiac muscle cells mediated by the sympathetic nervous system
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摘要: 目的: 观察丹参酮ⅡA(STS)对交感神经系统介导的心肌细胞炎症反应的作用研究。方法: 原代培养大鼠心肌细胞,使用不同浓度STS干预血管紧张素Ⅱ(Ang Ⅱ)刺激的心肌炎症反应,Real-time PCR及ELISA方法测定心肌细胞TLR4、TNF-α mRNA表达及细胞培养上清液TNF-α浓度,Western blot检测细胞浆蛋白IκB-α及细胞核蛋白NF-κB p65表达。结果: 不同浓度STS干预后,心肌细胞TLR4、TNF-α mRNA表达及TNF-α浓度下降,心肌细胞浆蛋白IκB-α水平下降及核蛋白NF-κB p65水平升高,随着干预浓度的升高,这种作用逐渐增强。结论: STS对AngⅡ刺激心肌细胞引起的炎症反应有良好的保护作用,其机制与抑制细胞内NF-κB激活,减少炎性因子分泌有关。Abstract: Objective: To observe the effect of sodium tanshinone ⅡA sulfonate on the inflammatory reaction mediated by the sympathetic nervous system in cardiomyocytes. Method: The primary myocardial cells were cultured in vitro.Different concentration of tanshinone ⅡA were performed to intervene the inflammatory response induced by angiotensin Ⅱ.The expression of TLR4 and TNF-α mRNA were detected by real time PCR,and the level of TNF-α was measured by ELISA,and the expression of IκBα and NF-κB p65 were detected by Western blot. Result: The expression of TLR4 and TNF-a mRNA in cardiomyocytes,as well as the concentration of TNF-α in cell culture supernanant decreased.And the levels of myocardial plasma protein I kappa B alpha decreased and the levels of nuclear protein NF-kappa B p65 increased after application of the different concentration of tanshinone ⅡA,and this effect was in a concentration-dependent manner. Conclusion: Sodium tanshinone ⅡA sulfonate has a positive effect on the inflammatory response induced by Ang Ⅱ stimulation in cardiomyocytes,and its mechanism might related to the inhibition of NF-κB activation and the depression of secretion of inflammatory cytokines in the cells.
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Key words:
- cardiomyocytes /
- sodium tanshinoneⅡA sulfonate /
- angiotensin Ⅱ /
- tumor necrosis factor
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