缺血后处理对大鼠肾缺血再灌注损伤的作用

梁永会; 陈平; 韩继媛

doi:10.13201/j.issn.1009-5918.2012.02.027

缺血后处理对大鼠肾缺血再灌注损伤的作用

- 1. 三峡大学第一临床医学院宜昌市中心人民医院急诊科(湖北宜昌, 443003);
- 2. 华中科技大学同济医学院附属协和医院急诊科

详细信息

通讯作者: 韩继媛,E-mail:jiyuanhan@126.com

中图分类号: R692

收稿日期: 2011-09-06

Effect of ischemic postconditioning on the renal ischemia-referfusionreperfusion injury in rats

- 1. Department of Emergency, Yichang Central People's Hospital, Yichang 443003, China;
- 2. Department of Emergency, Xiehe Hospital of Tongji Medical College, Huazhong University of Science and Technology

More Information

Corresponding author: HAN Jiyuan, E-mail: jiyuanhan@126.com

Received Date: 06 September 2011

摘要

摘要: 目的：成功建立肾脏缺血后再灌注损伤大鼠模型，研究缺血后处理对肾脏的保护作用。方法：夹闭大鼠左肾动静脉复制大鼠肾缺血再灌注损伤模型。雄性Wistar大鼠30只随机分成3组：缺血再灌注组（I/R，n=10），缺血后处理组（IPo，n=10），假手术组（S，n=10）。检测血肌酐浓度（Cr）、尿素氮（BUN），检测肾组织中超氧化物歧化酶（SOD）活性；取肾组织做HE染色，光镜下观察肾组织病理学变化。结果：S组血清肌酐水平为（63.83±17.26）μmol/L，血清尿素氮水平为（11.56±2.75） mmol/L。I/R组分别达到（175.94±64.53）μmol/L、（42.85±14.67） mmol/L，与S组相比差异有统计学意义（P<0.05），IPo组血清肌酐及尿素氮水平分别为（91.51±35.67）μmol/L、（15.91±4.12） mmol/L，与I/R组比较差异有统计学意义（P<0.05），而与S组比较差异无统计学意义（P>0.05）；S组肾组织中SOD含量为（91.3±2.9） U/mgport，I/R组为（55.3±1.6） U/mg-port，与S组比较差异有统计学意义（P<0.05），IPo组SOD含量为（71.6±2.7） U/mgport，SOD活性较I/R组升高，差异有统计学意义（P<0.05）；S组肾小球、肾小管未发现明显的形态学改变，I/R组病理形态与S组相比有显著差异，IPo组病理形态较I/R组明显减轻，统计学上有显著性差异。结论：缺血后处理对肾起保护作用，机制与增强了肾的抗氧化能力，减轻炎性细胞浸润有关。
- 肾缺血 /
- 缺血后处理 /
- 再灌注损伤
Abstract: Objective: To establish a model of renal ischemia-reperfusion in rats.To investigate the effect of ischemic postconditioning on the renal ischemia-reperfusion(I/R) injury and its mechanism.Method: The model of rat's RIRI was established,30 wistar rats were randomly divided into 3 groups:false operating group(S),ischemia reperfusion group(I/R) and ischemic postconditioning(IPo).Blood urea nitrogen(BUN),creatinine(Cr),superoxide dismutase(SOD) werewas ditecteddetected in each groups.and renal histopathology lesions were examined.Result: In I/R group,BUN and SCr serum levels increased significantly after ischemia reperfusion compared with false operating group(P<0.05),while BUN and SCr serum levels of IPo group decreased significantly after ischemic postconditioning compared with I/R group,there was statistic significance(P<0.05).in I/R group,SOD serum levels decreased significantly after ischemia reperfusion compared with false operating group(P<0.05),while SOD serum levels of IPo group increased significantly after ischemic postconditioning compared with I/R group,there was statistic significance(P<0.05).in S group,gomeruli and tubules have no obvious pathological changes,in I/R group,there are significant distinction of the pathological scale which compared with S group,in IPo group,the histologic damage induced by I/R were significantly ameliorated,there were statistic significance(P<0.05).Conclusion: Ischemic postconditioning play an important protective role in the renal ischemia-reperfusion injury,the mechanism is that which enhance kidney antioxidant capacity and reduce infiltration of inflammatory cells.
- renal ischemia /
- ischemic postconditioning /
- reperfusion injury

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参考文献(6)

[1]	范治璐,杜鑫,李卫平.缺血后处理对大鼠肾脏缺血再灌注损伤及NF-κB表达的影响[J].大连医科大学学报,2009,31(5):525-528.
[2]	陈光磊,王汉民,刘广厚,等.大鼠急性肾脏缺血再灌注后处理动物模型的建立[J].第四军医大学学报,2007,28(9):812-814.
[3]	THIBAULT H,PIOT C,STAAT P,et al.LongTerm Benefit of Postconditioning[J].Circulation,2008,117:1037-1044.
[4]	冷玉芳,姚爱军.肢体缺血后处理和肾脏缺血后处理对大鼠肾脏缺血-再灌注损伤的影响[J].临床麻醉学杂志,2009,25(11):974-976.
[5]	李爱芝,马加海,丁永波,等.七氟醚对大鼠急性肾缺血-再灌注损伤的保护作用[J].临床麻醉学杂志,2008,24(8):690-692.
[6]	LICHY,ROBERT M.Reactive sepecies mechanisms of cellular hypoxia-reoxygenation injury[J].Am J Physiol Cell Physiol,2002,282:227-241.