肾上腺素与血管加压素对窒息性心脏骤停大鼠早期心肺复苏的影响

郭一尘; 李瑞琳; 褚沛

doi:10.13201/j.issn.1009-5918.2013.10.006

肾上腺素与血管加压素对窒息性心脏骤停大鼠早期心肺复苏的影响

- 1. 兰州大学第一医院急诊科(兰州, 730000);
- 2. 兰州市第一人民医院神经内科

详细信息

通讯作者: 郭一尘,E-mail:sherilang@163.com

中图分类号: R541.7

收稿日期: 2013-03-15

Effect of epinephrine and vasopressin during early cardiopulmonary resuscitation in a mouse model of asphyxial cardiac arrest

- 1. Emergency Department of the First Hospital of Lanzhou University, Lanzhou 730000, China;
- 2. Department of Neurology, the First Hospital of Lanzhou

More Information

Corresponding author: GUO Yichen, E-mail: sherilang@163.com

Received Date: 15 March 2013

摘要

摘要: 目的：比较肾上腺素与血管加压素对窒息性心脏骤停大鼠早期心肺复苏的影响。方法：健康SD大鼠48只，体重200~250 g，雌雄不拘，将大鼠随机分成4组（n=12）：正常对照组（C组）、模型组（M组）、肾上腺素组（E组）和血管加压素组（V组）。M组、E组及V组大鼠均经气管夹闭1 min，建立窒息性心脏骤停模型。当窒息时间达1 min时，分别在常规心肺复苏前：C组和M组大鼠经股静脉注射生理盐水1 ml，E组及V组大鼠经股静脉分别注射肾上腺素0.04 mg/kg及血管加压素0.4 u/kg，同时开始胸外心脏按压及机械通气，观察自主循环恢复情况，5 min无效则放弃复苏。自主循环恢复的大鼠连续监测心电和血压30 min，记录在CPR 30 min内自主循环恢复情况及血流动力学；记录CPR 30 min后，采用免疫组织化学法检测各组实验大鼠血清中肿瘤坏死因子-水平、白细胞介素-6及白细胞介素-10水平。结果：E、V组大鼠自主循环恢复率均显著高于M组（分别为73.4%，74.6%，和15.3%，P<0.05），E组大鼠自主循环恢复率与V组比较差异无统计学意义（P>0.05）；E、V组大鼠平均动脉压明显高于M组大鼠（P<0.05），V组大鼠MAP高于E组大鼠，差异有统计学意义（P<0.05）。M组大鼠血清中白细胞介素-6及肿瘤坏死因子-水平与E、V组差异有统计学意义（P<0.05）；E组大鼠血清中肿瘤坏死因子-及白细胞介素-6水平高于V组（P<0.05）。E组大鼠血清中白细胞介素-10水平低于V组（P<0.05）。结论：肾上腺素与血管加压素在窒息性心脏骤停大鼠早期心肺复苏过程中复苏成功率无明显差异，但血管加压素可维持大鼠复苏后平均动脉压在相对较高水平；同时血管加压素可提高复苏后大鼠血清中抗炎因子水平。
- 心脏骤停 /
- 心肺复苏 /
- 肾上腺素 /
- 血管加压素 /
- 大鼠
Abstract: Objective: The purpose of this study was to compare the effects of epinephrine and vasopressin during early cardiopulmonary resuscitation (CPR) in a mouse model of asphyxial cardiac arrest.Method: Forty-eight SD rats of both sexes weighing 200~250 g were randomized into 4 groups(n=12 each) and receive saline (C,M-gro,1 ml i.v),epinephrine group(E-gro,0.04 mg/kg i.v),vasopressin group (V-gro,0.4 u/kg i.v),then CPR was initiated.Restoration of spontaneous circulation (ROSC) was observed.When there was a failure to restore spontaneous circulation after 5 min,resuscitation efforts were discontinued.Electrocardiogram and blood pressure of resuscitated animals were invasively monitored for an additional 30 minutes.ROSC and hemodynamics were observed and immediately recorded.Immunohisto chemistry technique was used to detect interleukin-6,interleukin-10 and tumor necrosis factor-levels.Result: Within 30 minutes of restoration of spontaneous circulation,rates of ROSC in E-gro and V-gro were significantly higher than in M-gro (73.4%,74.6%,和15.3%,P<0.05),there was no obvious difference between E-gro and V-gro(P>0.05).MAP in E-gro and V-gro were significantly higher than that in M-gro(P<0.05).MAP in V-gro was higher than in E-gro(P<0.05).The levels of interleukin-6 and tumor necrosis factor-were higher in E-gro than those in V-gro(P<0.05);The level of interleukin-10 was higher in V-gro than that in E-gro(P<0.05).Conclusion: The success rates of vasopressin and epinephrine are similar in early cardiopulmonary resuscitation,but vasopressin can maintain a high-level mean arterial pressure after resuscitation,and increase the level of anti-inflammatory factor.
- cardiac arrest /
- cardiopulmonary resuscitation /
- epinephrine /
- vasopressin /
- rat

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