稳定期COPD患者血清miR-181a与炎性因子的关系及对急性加重和死亡的预测价值

李翠娟; 唐颖; 慕丽娜; 彭翠兰

doi:10.13201/j.issn.1009-5918.2020.07.011

稳定期COPD患者血清miR-181a与炎性因子的关系及对急性加重和死亡的预测价值

- 大庆龙南医院(齐齐哈尔医学院第五附属医院)呼吸内科(黑龙江大庆, 163453)

详细信息

通讯作者: 李翠娟,E-mail:juanli085338@163.com

中图分类号: R541.4

收稿日期: 2020-03-14

Relationship between serum miR-181a and inflammatory cytokines in stable COPD patients and its predictive value for acute exacerbation and death

- Department of Respiratory Medicine, Longnan Hospital of Daqing, Daqing, Heilongjiang, 163453, China

More Information

Corresponding author: LI Cuijuan, E-mail: juanli085338@163.com

Received Date: 14 March 2020

摘要

摘要: 目的:研究稳定期慢性阻塞性肺疾病(COPD)患者血清miR-181a与炎性因子的关系及对急性加重和死亡的预测价值。方法:选择2016-09—2018-06期间在我院就诊的120例稳定期COPD患者作为研究的稳定期COPD组,同期体检且一般资料匹配的80例健康志愿者作为研究的对照组。检测血清miR-181a的表达量及炎性因子(TNF-α、IL-6、IL-8)的含量,随访患者急性加重及死亡情况。采用ROC曲线分析miR-181a对急性加重及死亡的预测价值,采用Kaplan-Meier曲线分析miR-181a低表达及高表达患者急性加重及死亡的差异。结果:稳定期COPD组患者血清miR-181a的表达量明显低于对照组,血清TNF-α、IL-6、IL-8的含量明显高于对照组(P<0.05);稳定期COPD患者血清miR-181a表达量与TNF-α、IL-6、IL-8含量均呈负相关,相关系数分别为-0.726、-0.413、-0.323(P<0.05);与miR-181高表达稳定期COPD患者比较,miR-181低表达稳定期COPD患者第一次急性加重的中位时间、生存的中位时间均明显缩短(P<0.05);miR-181a预测稳定期COPD患者急性加重的最佳截点为0.575,预测稳定期COPD患者死亡的最佳截点为0.480。结论:稳定期COPD患者血清miR-181a减少与炎性因子释放增多有关且对急性加重和死亡均具有一定的预测价值。
- 稳定期慢性阻塞性肺疾病 /
- miR-181a /
- 炎性因子 /
- 急性加重 /
- 死亡 /
- 预测
Abstract: Objective: To study the relationship between serum miR-181 a and inflammatory cytokines in stable chronic obstructive pulmonary disease(COPD) patients and its predictive value for acute exacerbation and death. Method: One hundred and twenty stable COPD patients admitted in our hospital from September 2016 to June 2018 were selected as the stable COPD group, and 80 healthy volunteers with matched general data were selected as the control group. The expression of miR-181 a and the contents of inflammatory cytokines(TNF-α, IL-6, IL-8) were measured. ROC curve was used to analyze the predictive value of miR-181 a for acute exacerbation and death, and Kaplan-Meier curve was used to analyze the difference of acute exacerbation and death between patients with low and high expression of miR-181 a. Result: The expression of miR-181 a in the stable COPD group was significantly lower than that in the control group, and the contents of TNF-α, IL-6 and IL-8 in the stable COPD group were significantly higher than that in the control group(P<0.05); the expression of miR-181 a in the stable COPD group was negatively correlated with the contents of TNF-α, IL-6 and IL-8, and the correlation coefficients were-0.726,-0.413 and-0.323, respectively(P<0.05); compared with patients with high expression of miR-181 in the stable COPD group, the median time of the first acute exacerbation and the median time of survival of patients with low expression of miR-181 were significantly shorter(P<0.05); the best cut-off point of miR-181 a for predicting the acute exacerbation of stable COPD patients was 0.575, and the best cut-off point for predicting the death was 0.480.Conclusion: The decrease of serum miR-181 a in stable COPD patients is related to the increase of the release of inflammatory factors, and has a certain predictive value for acute exacerbation and death.
- stable chronic obstructive pulmonary disease /
- miR-181a /
- inflammatory factors /
- acute exacerbation /
- death /
- prediction

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参考文献(12)

[1]	Salimi S,Noorbakhsh F,Faghihzadeh S,et al.Expression of miR-15b-5p,miR-21-5p,and SMAD7 in Lung Tissue of Sulfur Mustard-exposed Individuals with Long-term Pulmonary Complications[J].Iran J Allergy Asthma Immunol,2019,18(3):332-339.
[2]	Zhang D,Lee H,Wang X,et al.A potential role of microvesicle-containing miR-223/142 in lung inflammation[J].Thorax,2019,74(9):865-874.
[3]	Du X,Wei J,Tian D,et al.miR-182-5p contributes to intestinal injury in a murine model of Staphylococcus aureus pneumonia-induced sepsis via targeting surfactant protein D[J].J Cell Physiol,2020,235(1):563-572.
[4]	粱珍珍,吕燕平,张艳莉,等.miR-181a参与调控香烟提取物诱导的NR8383肺泡巨噬细胞自噬紊乱与促炎因子的生成[J].中国病理生理杂志,2019,35(4):710-717.
[5]	Xie L,Wu M,Lin H,et al.An increased ratio of serum miR-21 to miR-181a levels is associated with the early pathogenic process of chronic obstructive pulmonary disease in asymptomatic heavy smokers[J].Mol Biosyst,2014,10(5):1072-1081.
[6]	中华医学会呼吸病学分会慢性阻塞性肺疾病学组.慢性阻塞性肺疾病诊治指南(2013年修订版)[J].中华结核和呼吸杂志,2013,36(4):255-264.
[7]	Keller A,Ludwig N,Fehlmann T,et al.Low miR-150-5p and miR-320b Expression Predicts Reduced Survival of COPD Patients[J].Cells,2019,8(10):E1162.
[8]	Chen BB,Li ZH,Gao S.Circulating miR-146a/b correlates with inflammatory cytokines in COPD and could predict the risk of acute exacerbation COPD[J].Medicine(Baltimore),2018,97(7):e9820.
[9]	He H,Wang H,Pei F,et al.MiR-543 Regulates the Development of Chronic Obstructive Pulmonary Disease by Targeting Interleukin-33[J].Clin Lab,2018,64(7):1199-1205.
[10]	Majd AMM,Faghihzadeh S,Pourfarzam S,et al.Serum and sputum levels of IL-17,IL-21,TNFα and mRNA expression of IL-17 in sulfur mustard lung tissue with long term pulmonary complications(28 years after sulfur mustard exposure)[J].Int Immunopharmacol,2019,76():105828.
[11]	Zeng YY,Hu WP,Zuo YH,et al.Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD[J].Int J Chron Obstruct Pulmon Dis,2019,3(14):163-168.
[12]	Fermont JM,Masconi KL,Jensen MT,et al.Biomarkers and clinical outcomes in COPD:a systematic review and meta-analysis[J].Thorax,2019,74(5):439-446.