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摘要: 目的：观察维生素C对自发性高血压大鼠（SHR）血压的影响及血清一氧化氮、血浆内皮素1、血浆血管紧张素Ⅱ（AngⅡ）含量的影响。方法：选购10周龄SHR随机分为SHR-0组、SHR-1组、SHR-2组，每组各30只；WKY大鼠8只。1周后SHR-0组及WKY组分别10 mL蒸馏水灌胃，SHR-1组10 mL 2.5%维生素C溶液灌胃，SHR-2组2 ml 5%维生素C溶液灌胃，10 ml均在10~15 min内完成重复灌胃，分别比较实验干预前、持续给药1、2、4、8、12周后4组不同时间的血压变化；干预前和干预12周后随机选取8只SHR大鼠及4只WKY大鼠，比较各组血清一氧化氮、血浆内皮素1、血浆AngⅡ变化。结果：在实验干预前，SHR收缩压高于同龄WKY组（P<0.05），舒张压差异无统计学意义（P>0.05）。与SHR-0比较，SHR-1持续给药第2周末收缩压降低（P<0.05）；SHR-2持续给药第1至12周末舒张压、第2至12周末收缩压降低（P<0.05）。与SHR-1组比较，SHR-2在持续给药第2至12周末收缩压降低（P<0.05），第1、第4至12周末舒张压降低（均P<0.05）。WKY组在干预前及干预12周后血清一氧化氮、血浆内皮素1、血浆AngⅡ差异无统计学意义（P>0.05）。与WKY组相比，SHR干预前血清一氧化氮降低，血浆内皮素1、血浆AngⅡ增多（均P<0.05）。与WKY相比，干预12周后SHR-0、SHR-1血清一氧化氮降低，血浆内皮素1升高；SHR-0血浆AngⅡ升高。干预12周后与SHR-0组相比，SHR-1、SHR-2血清一氧化氮上升，血浆内皮素1、血浆AngⅡ下降（均P<0.05）。干预12周后与SHR-1组相比，SHR-2血清一氧化氮上升，血浆内皮素1下降（P<0.05）。结论：维生素C延缓了自发性高血压大鼠的血压升高，抑制了血清一氧化氮的减少及血浆内皮素1、血浆AngⅡ的增多。Abstract: Objective: To observe the efficacy of vitamin C on blood pressure and the changed content of serum nitric oxide(NO),plasma endothelin-1(ET-1) and plasma angiotensin Ⅱ(AngⅡ) in spontaneously hypertensive rats(SHR).Method: Ten weeks of SHR were randomly divided into SHR-0,SHR-1 and SHR-2,30 respectively,8 WKY rats as a group.After one week 10 ml distilled water was injected into stomach in the SHR-0 and WKY group,10 ml 2.5% and 5% vitamin C were respectively done in the SHR-1 and SHR-2 group.10 ml was finished repeated gavage in approximately 10-15 minutes.Blood pressure (systolic and diastolic pressure) was measured before the drug intervention and on the first weekend,the second weekend,the fourth weekend,the eighth weekend and the twelfth weekend after the continued intervention in each group.The changes were compared among four groups.The content of serum NO,plasma ET-1 and plasma AngⅡ was respectively measured and compared the changes with 8 SHR and 4 WKY that were randomly selected before the experiment and after 12 weeks of continued intervention.Result: Before the experimental intervention,the systolic pressure in all SHR was higher than WKY(P<0.05),but the diastolic blood pressure between the two showed no significance (P>0.05).Compared with SHR-0,the systolic blood pressure in SHR-1 decreased on the second weekend after the continued intervention (P<0.05),in the rest time of the continued intervention the systolic and diastolic blood pressure did not change significantly.However the systolic blood pressure in SHR-2 began to decrease after the two weeks of continued intervention (P<0.05) and the diastolic blood pressure in SHR-2 began to decrease after one week of continued intervention (P<0.05).Compared with SHR-1,the systolic blood pressure decreased after two weeks of continued intervention (P<0.05) and the diastolic blood pressure began to decrease on the first weekend and after four weeks of continued intervention(P<0.05) in SHR-2,but the diastolic blood pressure did not significantly change on the second weekend.Serum NO,plasma ET-1 and plasma Ang Ⅱ had no statistical significance before and after twelve weeks of intervention in WKY (P>0.05).Compared with WKY,serum NO decreased in SHR before intervention,however plasma ET-1 and plasma Ang Ⅱ increased (P<0.05).Compared with WKY,serum NO decreased and plasma ET-1 increased in SHR-0 and SHR-1 after twelve weeks of intervention,however plasma AngⅡ increased in SHR-0.Compared with SHR-0,serum NO increased and plasma ET-1,plasma AngⅡ decreased in SHR-1 and SHR-2 (P<0.05) after twelve weeks of intervention.Compared with SHR-1,serum NO increased and plasma ET-1 decreased in SHR-2 (P<0.05) after twelve weeks of intervention.Conclusion: Vitamin C delayed the increased blood pressure and inhibit the decrease of serum NO and the increase of plasma ET-1,plasma AngⅡ in spontaneously hypertensive rats.
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Key words:
- ascorbic acid /
- spontaneously hypertension /
- hypertension
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