Expression of TNF-α and TIPE2 in peripheral blood of patients with acute pancreatitis and their relationship with disease severity
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摘要: 目的:检测肿瘤坏死因子-α(TNF-α)、肿瘤坏死因子-α诱导蛋白8样分子2(TIPE2)在急性胰腺炎(AP)患者外周血中的表达,并探讨其与疾病严重程度的关系。方法:选取2018年3月-2019年9月期间我院消化内科收治的AP患者124例作为研究对象,依据AP亚特兰大共识将患者分为轻症组(68例)和重症组(56例),并应用Balthazar CT严重指数(CTSI)评分评估疾病严重程度;另选取同期在本院进行体检的健康人80例作为对照组。收集受试者一般资料。实时荧光定量PCR法(qRT-PCR)检测受试者血清TNF-α、TIPE2 mRNA水平。酶联免疫吸附法(ELISA)检测受试者血清TNF-α、TIPE2表达水平。Pearson法分析TNF-α、TIPE2、CTSI的相关性。多元线性回归分析AP患者CTSI评分的影响因素。结果:与对照组相比,轻症组和重症组TNF-αmRNA及血清水平依次升高(P<0.05),TIPE2 mRNA及血清水平依次降低(P<0.05)。Pearson法分析显示,TNF-α与TIPE2呈负相关(r=-0.612,P<0.05);TNF-α与CTSI呈正相关(r=0.662,P<0.05);TIPE2与CTSI呈负相关(r=-0.636,P<0.05)。多因素Logistic回归分析显示,TNF-α水平及CTSI评分偏高、TIPE2水平偏低是影响AP患者发生重症AP的危险因素(P<0.05)。多元线性回归分析显示,血清TNF-α、TIPE2对CTSI评分值的影响具有统计学意义(P<0.05)。结论:AP患者TNF-αmRNA及血清表达水平升高,TIPE2 mRNA及血清表达水平降低,与AP严重程度有关。
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关键词:
- 急性胰腺炎 /
- 肿瘤坏死因子-α /
- 肿瘤坏死因子-α诱导蛋白8样分子2
Abstract: Objective:To detect the expression of tumor necrosis factor-α(TNF-α) and tumor necrosis factor-α-inducible protein 8-like molecule 2(TIPE2) in peripheral blood of patients with acute pancreatitis(AP), and to explore their relationship with the severity of the disease.Methods:One hundred and twenty-four patients with AP admitted to the digestive department of our hospital from March 2018 to September 2019 were selected as the research objects. According to the Atlanta consensus of AP, the patients were divided into mild group(68 cases) and severe group(56 cases). The severity of disease was evaluated by Balthazar CT severity index(CTSI); another 80 healthy people who had physical examination in our hospital at the same time were selected as the control group. General data of the subjects were collected. The serum levels of TNF-α and TIPE2 mRNA were detected by real-time fluorescence quantitative PCR(qRT-PCR). The serum levels of TNF-α and TIPE2 were detected by enzyme-linked immunosorbent assay(ELISA). Pearson method was used to analyze the correlation among TNF-α, TIPE2 and CTSI. Multiple linear regression analysis was used to analyze the influencing factors of CTSI score in AP patients.Results:Compared with those in the control group, levels of serum TNF-α mRNA and protein in mild and severe groups were increased in turn(P<0.05), levels of serum TIPE2 mRNA and protein decreased in turn(P<0.05). Pearson analysis showed that TNF-α was negatively correlated with TIPE2(r=-0.612, P<0.05); TNF-α was positively correlated with CTSI(r=0.662, P<0.05); TIPE2 was negatively correlated with CTSI(r=-0.636, P<0.05). Multivariate Logistic regression analysis showed that the high level of TNF-α, CTSI score and low level of TIPE2 were the risk factors for severe AP. Multiple linear regression analysis showed that serum TNF-α and TIPE2 had significant effect on CTSI score(P<0.05).Conclusion:The levels of serum TNF-α mRNA and protein are increased in AP patients, while the levels of serum TIPE2 mRNA and protein are decreased, and they are related to the severity of AP. -
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