MMP-9及MCP-1在单纯疱疹病毒性脑炎患者脑脊液中的表达及意义

周瑜; 曾艳平; 罗瑛; 翁超; 卢祖能

doi:10.13201/j.issn.1009-5918.2020.03.002

MMP-9及MCP-1在单纯疱疹病毒性脑炎患者脑脊液中的表达及意义

- 武汉大学人民医院神经内科(武汉, 430060)
基金项目:
国家自然科学基金青年科学基金项目(No:81701196)

详细信息

通讯作者: 周瑜,E-mail:zhouyu99131@126.com

中图分类号: R512.3

收稿日期: 2019-09-01

Expression of MMP-9 and MCP-1 in cerebrospinal fluid of patients with herpes simplex encephalitis

- Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, 430060, China

More Information

Corresponding author: ZHOU Yu, E-mail: zhouyu99131@126.com

Received Date: 01 September 2019

摘要

摘要: 目的:观察单纯疱疹病毒性脑炎(HSE)患者脑脊液中MMP-9与MCP-1的浓度变化并探讨其意义。方法:选择HSE患者30例(HSE组),非HSE患者28例为对照组,应用ELISA法检测血清和脑脊液中MMP-9与MCP-1浓度。患者脑脊液以及加入MMP-9抑制剂SB-3CT(10μmol/L)的脑脊液分别作用于海马脑片后,TUNEL染色检测神经细胞凋亡。结果:HSE组脑脊液中MMP-9和MCP-1含量均显著高于对照组(P<0.01)。并且MMP-9与MCP-1的表达水平与脑脊液中白细胞及蛋白含量均呈正相关。抑制脑脊液中MMP-9表达可以减少神经细胞的凋亡。结论:脑脊液中MMP-9与MCP-1参与了HSE的病理生理过程,可以作为HSE的临床诊断与干预指标。
- 单纯疱疹病毒性脑炎 /
- 脑脊液 /
- 基质金属蛋白酶9 /
- 单核细胞趋化蛋白-1
Abstract: Objective:To investigate MMP-9 and MCP-1 levels in cerebrospinal fluid of patients with herpes simplex encephalitis, and to explore the clinical relationship. Method:Serum and cerebrospinal fluid MMP-9 and MCP-1 concentrations in serum and cerebrospinal fluid were detected by enzyme-linked immunosorbent assay in 30 cases with herpes simplex encephalitis(HSE group), and were compared to those in 28 cases with no HSE for control. After the patients' cerebrospinal fluid and those added with MMP-9 inhibitor SB-3 CT(10 μmol/L) were applied to the hippocampal slices. The apoptosis of neurons was detected by TUNEL staining. Result:The CSF levels of MMP-9 and MCP-1 in HSE patients were obviously higher than those in the control group(P<0.01), and both of the expressions of MMP-9 and MCP-1 were positively correlated with the cell and protein levels in CSF(P<0.01). Inhibition of MMP-9 expression can reduce neuronal apoptosis.Conclusion:MMP-9 and MCP-1 in cerebrospinal fluid participated in the pathophysiological process of HSE, and may be used as potential markers for clinical diagnosis of HSE.
- herpes simplex encephalitis /
- cerebrospinal fluid /
- MMP-9 /
- MCP-1

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参考文献(12)

[1]	Rabinstein AA.Herpes Virus Encephalitis in Adults:Current Knowledge and Old Myths[J].Neurol Clin,2017,35(4):695-705.
[2]	Modi S,Mahajan A,Dharaiya D,et al.Burden of herpes simplex virus encephalitis in the United States[J].J Neurol,2017,264(6):1204-1208.
[3]	Koyanagi N,Imai T,Shindo K,et al.Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis[J].J Clin Invest,2017,127(10):3784-3795.
[4]	Bradshaw MJ,Venkatesan A.Herpes Simplex Virus-1 Encephalitis in Adults:Pathophysiology,Diagnosis,and Management[J].Neurotherapeutics,2016,13(3):493-508.
[5]	Zhou Y,Zeng YP,Zhou Q,et al.The effect of cyclin-dependent kinases inhibitor treatment on experimental herpes simplex encephalitis mice[J].Neurosci Lett,2016,627:71-76.
[6]	Brilha S,Ong CWM,Weksler B,et al.Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis[J].Sci Rep,2017,7(1):16031.
[7]	Chaturvedi M,Kaczmarek L.Mmp-9 inhibition:a therapeutic strategy in ischemic stroke[J].Mol Neurobiol,2014,49(1):563-573.
[8]	Sellner J,Simon F,Meyding-Lamade U,et al.Herpes-simplex virus encephalitis is characterized by an early MMP-9 increase and collagen type Ⅳ degradation[J].Brain Res,2006,1125(1):155-162.
[9]	Zhou Y,Zeng YP,Zhou Q,et al.The effect of captopril on the expression of MMP-9 and the prognosis of neurological function in herpes simplex encephalitis mice[J].Neurol Res,2016,38(8):733-739.
[10]	Kirkley KS,Popichak KA,Afzali MF,et al.Microglia amplify inflammatory activation of astrocytes in manganese neurotoxicity[J].J Neuroinflammation,2017,14(1):99.
[11]	Al-Obaidi MMJ,Desa MNM.Mechanisms of Blood Brain Barrier Disruption by Different Types of Bacteria,and Bacterial-Host Interactions Facilitate the Bacterial Pathogen Invading the Brain[J].Cell Mol Neurobiol,2018,38(7):1349-1368.
[12]	Uyangaa E,Choi JY,Patil AM,et al.Dual TLR2/9 Recognition of Herpes Simplex Virus Infection Is Required for Recruitment and Activation of Monocytes and NK Cells and Restriction of Viral Dissemination to the Central Nervous System[J].Front Immunol,2018,9:905.