Establishment of obesity rat model with acute necrotic pancreatitis and evaluation for liver injury
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摘要: 目的:建立肥胖大鼠急性坏死性胰腺炎(ANP)模型并观察肝脏损伤特点。方法:40只雄性SD大鼠随机分为普通饲料组(N组)和高脂饲料组(H组),每组20只,分别采用普通饲料和高脂饲料喂养8周并监测体重。然后分别将N组和H组大鼠随机分为对照组(N-control组,H-control组,n=10)和ANP组(N-ANP组,H-ANP组,n=10)。ANP组大鼠通过胰胆管逆行注射牛磺胆酸钠方法建立ANP模型,术后6 h剖杀取材。全自动生化仪测量血清淀粉酶(AMY)、脂肪酶(LIPA),血清甘油三脂(TG)、总胆固醇(TC),谷丙转氨酶(ALT)、和谷草转氨酶(AST)水平,苏木精-伊红切片观察肝脏和胰腺组织病理变化。结果:高脂饲料喂养8周后大鼠体重明显增加(P<0.05),腹腔脂肪组织增多,同时并发脂肪肝和高脂血症。联合胰胆管逆行注射牛磺胆酸钠方法建立ANP模型后肥胖大鼠腹腔可见明显皂化斑及肝脏坏死灶,血清转氨酶水平以及肝脏病理评级较非肥胖大鼠明显增高(P<0.05)。结论:高脂饲料喂养联合胰胆管逆行注射法可建立肥胖大鼠ANP模型,肥胖大鼠发生ANP时肝脏损伤程度更重。Abstract: Objective:To establish a model of obesity rat with acute necrotic pancreatitis (ANP) and observe the characteristics of liver injury.Method:Forty male SD rats were firstly divided into normal diet group (N group,n=20) and high fat diet group (H group,n=20) randomly.After 8 weeks of feeding with standard and high fat diet separately,the rats in N group and H group were randomly divided into sham operation group (N-control/H-control group,n=10) and acute necrotic pancreatitis (N-ANP/H-ANP group,n=10) separately.ANP model was induced by retrograde infusion of 5% sodium taurocholate saline solution into biliopancreatic duct,and rats in each group were sacrificed at 6 hours after the operation.The levels of serum amylase (AMY),lipase (LIPA),triglyceride (TG),total cholesterol (TC),alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using automatic biochemistry analyzer.Pathological changes of pancreas and liver tissues were examined and pathological scores were recorded.Result:After 8 weeks,the body weights of high fat diets group rats were significantly higher than normal group (P<0.05).There is well marked increase and accumulation of intra abdominal fat in H group and the rats were complicated by fatty liver and hyperlipemia.Plenty of saponification and adiponecrosis could be found in H-ANP group rats and the concentration of serum ALT,AST in addition to the histological grading of liver injury were significantly higher than N-ANP group (P<0.05).Conclusion:High fat diets coupled with operations can successfully establish a model of obesity rat with acute necrotic pancreatitis.Liver injury is more critical during the process of ANP especially in obesity rats.
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Key words:
- obesity /
- acute necrotic pancreatitis /
- model /
- liver injury
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