山莨菪碱对心脏骤停猪肺氧化应激及细胞凋亡的影响

张杰; 刘亚华; 代正; 周满红; 尹雪莲; 李玉华; 沈洪

doi:10.13201/j.issn.1009-5918.2015.12.017

山莨菪碱对心脏骤停猪肺氧化应激及细胞凋亡的影响

- 1. 中国人民解放军总医院急诊科(北京, 100853);
- 2. 武警总医院急救医学中心;
- 3. 四川省人民医院急诊科;
- 4. 遵义医学院附属医院干部保健科;
- 5. 北京同仁医院急诊科;
- 6. 胜利油田中心医院急诊科

详细信息

通讯作者: 沈洪,E-mail:shenhong@em120.com

中图分类号: R541

收稿日期: 2015-10-14

Affect of anisodamine on lung injury induced by Oxidative stress and apoptosis in cardiac arrest swine

- 1. Department of Emergency, The General Hospital of PLA, Beijing 100853, China;
- 2. Department of Emergency, The General Hospital of Chinese People's Armed Police Forces;
- 3. Department of Emergency, Sichuan Provincial People's Hospital;
- 4. Cadre Ward, Affiliated Hospital of Zunyi Medical College;
- 5. Department of Emergency, Beijing Tongren Hospital;
- 6. Department of Emergency, Shengli Oilfield Central Hospital

More Information

Corresponding author: SHEN Hong, E-mail: shenhong@em120.com

Received Date: 14 October 2015

摘要

摘要: 目的：探讨山莨菪碱对心搏骤停（CA）猪肺氧化应激及细胞凋亡的影响。方法：选择健康雄性家猪23头，按随机数字表法分为对照组（n=5）、肾上腺素组（n=9）和山莨菪碱组（n=9），用右心室交流电致颤法建立CA模型。在基础时点、CA 8 min、自主循环恢复（ROSC）即刻、ROSC 30 min和ROSC 24 h留取血液标本，ROSC 24 h后处死动物留取肺组织标本。测定各时间点血浆及24 h时点肺组织的生化指标：黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活力，硫代巴比妥酸法测定丙二醛（MDA）含量。免疫组织化学法检测肺组织细胞色素C（Cyto-C）蛋白表达情况并用图像处理系统测定阳性反应物的免疫组织化学吸光度值（MOD值）。ELISA法检测肺组织Cyto-C和Caspase-3蛋白含量。Hoechest33258染色并计数测定肺细胞凋亡指数（AI）。结果：①复苏成功的动物均存活24 h，所有动物肺脏表面结构完整，未出现肋骨骨折刺伤肺脏的情况。②各时间点血浆SOD活力2复苏组组间比较差异无统计学意义（P>0.05）；而山莨菪碱组肺组织SOD活力明显高于肾上腺素组（P<0.05）。ROSC 30 min、ROSC 24 h山莨菪碱组血浆MDA含量较肾上腺素组减少（P<0.05），同时肺组织MDA含量明显低于肾上腺素组（P<0.01）。③复苏后2组肺组织Cyto-C表达均较对照组明显上调（P<0.01），阳性细胞主要分布于肺泡上皮细胞。山莨菪碱组Cyto-C呈弱阳性表达，肾上腺素组表达明显高于山莨菪碱组（P<0.01）。④肺组织Cyto-C、Caspase-3蛋白含量2复苏组间比较差异无统计学意义（P>0.05）。⑤2复苏组肺细胞AI均较对照组显著增加（P<0.01），山莨菪碱组较肾上腺素组降低（P<0.05）。结论：山莨菪碱可能通过抑制再灌注后脂质过氧化反应，以及抑制线粒体途径细胞凋亡途径从而参与了CA后肺缺血再灌注损伤的保护。
- 心搏骤停 /
- 心肺复苏 /
- 山莨菪碱 /
- 缺血再灌注损伤 /
- 氧化应激 /
- 细胞凋亡
Abstract: Objective: To investigate the affect of anisodamine on lung injury induced by Oxidative stress and apoptosis in cardiac arrest swine.Method: Twenty-three health male swines were divided into three groups using random digits table,sham group (n=5),epinephrine group (n=9) and anisodamine group (n=9).The cardiac arrest (CA) model was induced by alternating current.Lung tissue were harvested at 24 h after ROSC.The blood sample were took at basic time,CA 8min,ROSC,ROSC 30 min,ROSC 24 h.The blood samples and lung specimens were repaired for analyzing the enzyme activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) respectively.The lung specimens were observed to assess cytochrome C (Cyto C) protein expression by immunohistochemistry.The lung sample contents of Cyto C and Caspase-3 were detected by ELISA.The lung tissue were stained with Hoechst 33258 to assess apoptosis.Result: ①All animals which achieved ROSC survived to 24 h.and lungs gross specimen surface were all completed without stabbing.② There were no difference in SOD activity between two CPR groups at the same point (P>0.05).The lung SOD activity of Ani+CA/R group was higher than CA/R group (P<0.05).MDA level of Ani+CA/R group was lower than CA/R group at ROSC 30 min,ROSC 24 h (P<0.05).The lung MDA content of Ani+CA/R group was lower than CA/R group (P<0.01).③The expression of lung tissue cytochrome C in both CPR group were significantly higher than control group (P<0.01).The positive cells were mainly distributed in alveolar epithelial cells.Cytochrome C of Ani+CA/R group were weakly positive expression and significantly lower than CA/R group (P<0.01).④were significantly higher than that of control group (P<0.01),There were no difference in Cyto C and Caspase-3 contents of lung tisssue between two CPR groups(P>0.05).⑤Lung cell apoptosis index of both CPR group were higher significantly than the control group (P<0.01).Compared with CA/R group,cell apoptosis index of Ani+CA/R group reduced.(P<0.05).Conclusion: Possibly through regulating oxygen radicals metabolism and affecting mitochondrial pathway of apoptosis pathway,Anisodamine relieved the lung injury after cardiac arrest.
- cardiac arrest /
- cardiopulmonary resuscitation /
- anisodamine /
- ischemia-reperfusion injury /
- Oxidative stress /
- apoptosis

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